Background: Gliomas are the most common primary malignant brain tumors in adults. It has a devastating impact on the cognitive, physical, social, and psychological well-being of patients. Informal caregivers refer to family members, friends, and other carers of the patient who provide unpaid care for patients. They provide physical and psychological support for patients and the family during the disease process. Despite this, there is a paucity of knowledge regarding the experiences and needs of glioma caregivers across the disease trajectory. Methods: A systematic review will be conducted to identify the experiences and needs of informal caregivers of patients with glioma. Seven English databases and four Chinese databases will be analyzed. The search is limited to peer-reviewed full-text articles published either in English or Chinese, with no restrictions on the publication period. According to the Joanna Briggs Institute (JBI) manual for evidence synthesis, two independent reviewers will apply the JBI Critical Appraisal Checklist for Qualitative Research to evaluate the methodological quality of each study. The JBI meta-aggregation method will subsequently be used to synthesize the data, eventually forming themes, categories, and findings. Discussion: The systematic review is expected to be the first qualitative synthesis of evidence pertains to the experience of family caregivers for glioma patients. The findings generated from the systematic review may be rewarding for researchers to improve care and quality of life for glioma patients and their family members. Trial registration: PROSPERO CRD42020222307.